mc 3t3 e1 cells Search Results


mouse osteoblastic cells  (ATCC)
97
ATCC mouse osteoblastic cells
Mouse Osteoblastic Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/NIH+3T3+mouse+CD40L%2F+mouse+BAFF/10__1039_slash_c5ra22178a-71-0-4
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mouse osteoblastic cells - by Bioz Stars, 2026-10
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nih3t3 cells  (MedChemExpress)
94
MedChemExpress nih3t3 cells
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
Nih3t3 Cells, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/Serpin+E1%2C+Mouse/10__1016_slash_j__eng__2024__08__014-107-6-19
Average 94 stars, based on 1 article reviews
nih3t3 cells - by Bioz Stars, 2026-10
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mc 3t3 e1  (ATCC)
96
ATCC mc 3t3 e1
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
Mc 3t3 E1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/MC3T3-E1+Subclone+14/ppr0551029-522-30-31
Average 96 stars, based on 1 article reviews
mc 3t3 e1 - by Bioz Stars, 2026-10
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mc 3t3 e1 preosteoblast cell line  (ATCC)
97
ATCC mc 3t3 e1 preosteoblast cell line
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
Mc 3t3 E1 Preosteoblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/TIBx%3B+Epithelial+liver%3B+Mouse/pmc07466474-85-0-5
Average 97 stars, based on 1 article reviews
mc 3t3 e1 preosteoblast cell line - by Bioz Stars, 2026-10
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nih3t3 e1 fibroblasts  (ATCC)
99
ATCC nih3t3 e1 fibroblasts
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
Nih3t3 E1 Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/NIH%2F3T3/pmc06562610-45-0-5
Average 99 stars, based on 1 article reviews
nih3t3 e1 fibroblasts - by Bioz Stars, 2026-10
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mc-3t3-e1 cell  (CH Instruments)
90
CH Instruments mc-3t3-e1 cell
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
Mc 3t3 E1 Cell, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/3t3+l1+adipocytes/pm28866215-250-0-12
Average 90 stars, based on 1 article reviews
mc-3t3-e1 cell - by Bioz Stars, 2026-10
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mouse embryo fibroblast cell line atcc crl 1658 mc3t3 e1  (ATCC)
99
ATCC mouse embryo fibroblast cell line atcc crl 1658 mc3t3 e1
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
Mouse Embryo Fibroblast Cell Line Atcc Crl 1658 Mc3t3 E1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/NIH%2F3T3%3B+Embryonic+Fibroblast%3B+Mouse/us09958436-389-41-46
Average 99 stars, based on 1 article reviews
mouse embryo fibroblast cell line atcc crl 1658 mc3t3 e1 - by Bioz Stars, 2026-10
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cell lines hek293t n a rrid cvcl 0063 nih 3t3 n a rrid cvcl 0594 plat e n a rrid cvcl b488 og2  (ATCC)
95
ATCC cell lines hek293t n a rrid cvcl 0063 nih 3t3 n a rrid cvcl 0594 plat e n a rrid cvcl b488 og2
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
Cell Lines Hek293t N A Rrid Cvcl 0063 Nih 3t3 N A Rrid Cvcl 0594 Plat E N A Rrid Cvcl B488 Og2, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/E1%2E4/pm35385732-488-106-131
Average 95 stars, based on 1 article reviews
cell lines hek293t n a rrid cvcl 0063 nih 3t3 n a rrid cvcl 0594 plat e n a rrid cvcl b488 og2 - by Bioz Stars, 2026-10
95/100 stars
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3t3 preosteoblasts  (ATCC)
97
ATCC 3t3 preosteoblasts
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
3t3 Preosteoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/MC3T3-E1+Subclone+4%3B+Preosteoblast%3B+Mouse/pmc04945899-82-12-14
Average 97 stars, based on 1 article reviews
3t3 preosteoblasts - by Bioz Stars, 2026-10
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cell line  (Procell Inc)
86
Procell Inc cell line
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
Cell Line, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/cell+line/10__1016_slash_j__colcom__2025__100864-40-3-12
Average 86 stars, based on 1 article reviews
cell line - by Bioz Stars, 2026-10
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imaris  (Oxford Instruments)
99
Oxford Instruments imaris
Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in <t>NIH3T3</t> cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.
Imaris, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc+3t3+e1+cells/Imaris/custom%40imaris%4040197239
Average 99 stars, based on 1 article reviews
imaris - by Bioz Stars, 2026-10
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Image Search Results


Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in NIH3T3 cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.

Journal: Engineering

Article Title: PAI-1 Derived from Alveolar Type 2 Cells Drives Aging-Associated Pulmonary Fibrosis

doi: 10.1016/j.eng.2024.08.014

Figure Lengend Snippet: Fig. 4. Impact of the secretome from senescent epithelial cells on fibrosis and Collagen I synthesis. (a) Schematic of Collagen I biosynthesis; (b) schematic diagram of the cell culture models used for conditioned medium culture and coculture experiments in this study; (c) Western blot analysis of senescence- and cell cycle-associated proteins in primary AT2 cells treated with 30 gL1 D-galactose for 48 h (n = 3); (d–e) Western blot analysis of fibrosis, collagen synthesis, and degradation-related proteins in NIH3T3 cells cultured with conditioned medium from D-galactose-induced senescent (d) primary AT2 cells and (e) A549 cells for 48 h (n = 3). MMP1: matrix metalloproteinase.

Article Snippet: After 8 h of transfection, the NIH3T3 cells were exposed to medium containing 2 lg mL 1 PAI-1 (HY-P71133, MCE) and 0.4% serum.

Techniques: Cell Culture, Western Blot

Fig. 5. PAI-1 secreted by senescent epithelial cells promotes collagen I synthesis via the TGF-b/Smad2/3 pathway. (a) Schematic diagram of the process used to screen for key SASP factors associated with fibrosis; (b) RT-qPCR analysis of key fibrosis-related SASP factors in 3 and 28 month old mouse lung tissues. Each group comprised six biological replicates and three technical replicates. Results are presented as mean ± standard deviation (SD); (c) expression analysis of key fibrosis-related SASP factors in primary AT2 cells from 3 and 24 month old mice (n = 3); (d) Western blot analysis of PAI-1 protein in mouse lung tissues of different ages and D-galactose-induced senescent A549 cells (n = 3); (e, f) Western blot analysis of fibrosis and collagen synthesis-related proteins in (e) NIH3T3 cells and (f) WI-38 cells stimulated with gradient concentrations of PAI-1 (0, 0.5, 1.0, and 2.0 lgmL1) for 48 h (n = 3); (g, h) activation of the TGF-b pathway in (g) NIH3T3 and (h) WI-38 cells after stimulation with 2.0 lgmL1 PAI-1 for 48 h (n = 3); (i) effect of PAI-1 on Col1a1 synthesis after TGF-b pathway inhibition with SB431542 (n = 3); (j) impact of PAI-1 on Col1a1 synthesis after silencing Smad2/3 with siSmad2/3 (n = 3).

Journal: Engineering

Article Title: PAI-1 Derived from Alveolar Type 2 Cells Drives Aging-Associated Pulmonary Fibrosis

doi: 10.1016/j.eng.2024.08.014

Figure Lengend Snippet: Fig. 5. PAI-1 secreted by senescent epithelial cells promotes collagen I synthesis via the TGF-b/Smad2/3 pathway. (a) Schematic diagram of the process used to screen for key SASP factors associated with fibrosis; (b) RT-qPCR analysis of key fibrosis-related SASP factors in 3 and 28 month old mouse lung tissues. Each group comprised six biological replicates and three technical replicates. Results are presented as mean ± standard deviation (SD); (c) expression analysis of key fibrosis-related SASP factors in primary AT2 cells from 3 and 24 month old mice (n = 3); (d) Western blot analysis of PAI-1 protein in mouse lung tissues of different ages and D-galactose-induced senescent A549 cells (n = 3); (e, f) Western blot analysis of fibrosis and collagen synthesis-related proteins in (e) NIH3T3 cells and (f) WI-38 cells stimulated with gradient concentrations of PAI-1 (0, 0.5, 1.0, and 2.0 lgmL1) for 48 h (n = 3); (g, h) activation of the TGF-b pathway in (g) NIH3T3 and (h) WI-38 cells after stimulation with 2.0 lgmL1 PAI-1 for 48 h (n = 3); (i) effect of PAI-1 on Col1a1 synthesis after TGF-b pathway inhibition with SB431542 (n = 3); (j) impact of PAI-1 on Col1a1 synthesis after silencing Smad2/3 with siSmad2/3 (n = 3).

Article Snippet: After 8 h of transfection, the NIH3T3 cells were exposed to medium containing 2 lg mL 1 PAI-1 (HY-P71133, MCE) and 0.4% serum.

Techniques: Quantitative RT-PCR, Standard Deviation, Expressing, Western Blot, Activation Assay, Inhibition